You can display the proteolytic enzyme site search results in a number of ways:
- as a list of enzymes that cut the sequence
- as a list of enzymes that did not cut the sequence
- as a proteolytic map
- as an annotated sequence
- as a list of the predicted fragments for each digest.
One or more of the displays can be generated at once.
Each display type can be saved to disk or printed when its window is active.
1. The Proteolytic Enzyme Analysis Display dialog box is displayed on completion of each analysis. To display this dialog box at other times, for example to change the display parameters, choose Analyze | Proteolytic Enzyme when any restriction analysis display result window is active.
2. Select the List cutters by check box to display a list of the enzymes that cut the sequence and the locations of the cuts.
Use the drop-down menu to choose how to order the list: by name, by position of cuts, or by number of cuts.
3. Select the List non-cutters check box to display a list of the enzymes that were considered in the search but were found not to cut the sequence.
4. Select the Proteolytic map check box to display the locations of the cut sites in a graphical form.
5. Select the Annotated sequence check box to display an annotated sequence display with the cut sites displayed at their proper locations along the sequence. The appearance of this display can be changed interactively by choosing Options | Format Annotated Display. For more information see Format annotated display.
6. Select the Frag predictions check box to display fragment predictions for the digests. The enzymes used are those that have satisfied the Filter Options.
Use the drop-down menu to choose from the following:
- single for single digests
- double for double digests
- both for single and double digests
- all combined for digests using up to six selected enzymes.
7. Select OK to display the results.
Searching for proteolytic sites