The proteolytic site search results can be filtered as follows:
- by the number of cuts an enzyme makes
- by cut site size
- by the residue range in which the cut site occurs.
1. The Proteolytic Enzyme Analysis Display dialog box is displayed on completion of each analysis. To display this dialog box at other times, for example to change the display parameters, choose Analyze | Proteolytic Enzyme when any proteolytic analysis display result window is active.
2. To restrict the displays to enzymes that cut the sequence a limited number of times, select the # of cuts checkbox and type a number from 1 to 20 to set the upper limit for number of cuts.
3. To restrict the search to enzymes whose recognition sites are a certain size, select the site size checkbox and type the size limits into the min and max text boxes. MacVector accepts values from 1 to 100.
4. To restrict the displays to enzymes that do not cut within a given region, select the no cuts checkbox, then either type in the numbers of the bases that bracket the region, or choose a region from the features table drop-down menu at the right of the text boxes.
5. To restrict the displays to enzymes that cut only within a given region, select the cuts only checkbox, then either type in the numbers of the bases that bracket the region, or choose a region from the features table drop-down menu at the right of the text boxes.
6. Select the Display Options as required.
7. Select OK to display the results.