If required, you can select a subset of the available enzymes from the file you are using. Before you perform the analysis, open the proteolytic enzyme file and click in front of the name of any enzyme you wish to select. A check mark appears to mark your choice. Save the proteolytic enzyme file to save the selections.
1. Make the required amino acid sequence window active.
2. Choose Analyze | Proteolytic Enzyme.
The Proteolytic Enzyme Analysis dialog box is displayed.
3. Select Enzyme File to choose the restriction enzyme file that will be used for the analysis.
A file selection dialog box is displayed, enabling you to choose the file.
4. Choose the required file from the scrolling list and select Choose.
5. You can restrict the search to a certain region of the sequence by typing in the sequence numbers that bracket the region in the Region panel, or by selecting a region from the features table drop-down menu at the right of the text boxes.
6. Select your search criteria by chosing from the Search Using drop-down menu as follows:
- choose all enzymes to use all the enzymes in the selected enzyme file
- choose selected enzymes to use only the enzymes that you have selected within the enzyme file.
7. Select OK to perform the analysis.
When the analysis is completed, the Proteolytic Enzyme Analysis Display dialog box is displayed.