How do I make a permanent note of where my primer binds on a sequence.

Once you have designed a pair of primers with MacVector, you can quickly annotate both the primers and product to your sequence template. The annotation contains a timestamp and the primer’s characteristics. It also contains the full sequence of the primer, which means any custom ends (with mismatches or new restriction sites) are also included.

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1. Design a pair of primers
2. Switch to the primer Map results tab
3. Select a primer and drag and drop it onto your template sequence.
4. You can also drag and drop the product.
5. Alternatively right click on the primer and select "Create Prim_bind"

See the Primer Design - Overview help topic for more details.

Related Topics.

Primer Design - Overview

How do I design a pair of primers to amplify a gene.

How do I? - videos

Adding primers to the Primer Database

Sequencing primers/probes

Test sequencing primer/probe