How do I subclone digested fragments into a vector

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1. Open a sequence
2. Select one restriction enzyme site in the Map tab.
3. Hold down [SHIFT} key and select a second site.
4. click DIGEST
5. In the Click Cloning window drag and drop your digested fragment and drop on your cloning vector window.

See the Click Cloning help topic for more details.

Related Topics.

Click Cloning

How do I? - videos

How to digest and ligate fragments into a vector

End modification

Gateway and TOPO cloning

Restriction enzyme

Resetting the origin of a circular sequence.

Automatic Restriction Enzyme Analysis