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How do I design a pair of primers to amplify a gene?

You can design a set of primers to amplify a gene in as little as three mouse clicks.

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  1. Open your sequence.
  2. Open the Map tab, and click on a feature.
  3. Go to ANALYZE | PRIMER DESIGN/TEST(PAIRS).
  4. Click OK.

See the Primer Design - Overview help topic for more details.

Related Topics.

Primer Design - Overview

How QuickTest Primer scores hairpin loops and primer dimers.

How do I? - videos

Adding primers to the Primer Database

Sequencing primers/probes