How do I design a primer to match an existing primer?

The Primer database allows you to store your own collection of primers. You can design new primers to match regularly used ones.

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1. Open your template sequence
2. Switch to the Map tab and select the region you want to amplify.
3. Go to ANALYZE | PRIMER DESIGN/TEST(PAIRS).
4. For the left primer click USE THIS Primer
5. Use the drop down menu to enter the existing primer from the primer database
6. Click OK

See the Primer Database help topic for more details and the Primer Design - Overview help topic for an overview of MacVector's primer design tools.

Related Topics.

Primer Design - Overview

How do I? - videos

Adding a primer to the Primer Database

How to design primers with tails and mismatches.

QuickTest Primers

Design Primers (Primer3)

Sequencing primers/probes

Test sequencing primer/probe