How do I generate a transcript of a DNA sequence.

The Generate Transcript dialog box lets you assemble a transcription sequence from segments defined by exon, intron, CDS or RNA features.

Choose Analyze | Generate Transcript.
From the Method drop-down menu, choose one of the following methods for selecting segments to transcribe.
- Single CDS
- Specify exons
- Specify introns
- Specify RNA sequences.
The appropriate features are displayed in a list. To enter a range in the sequence, type the appropriate sequence numbers in the From and to text boxes.
From the Strand menu, select plus or minus. If you have selected the Single CDS method, CDS features of both strands are listed and this menu is not available.
Do one of the following, depending on the transcription method you chose in step 2.
- Specify exons : To deselect any listed features that you do not want transcribed, click in their check boxes.
- Single CDS: Select a CDS feature for transcription from the list. The selected feature is highlighted.
- Specify introns or Specify RNA sequences:To select any listed introns that you want transcribed, click in their check boxes.
If you chose Specify exons or Specify RNA features, you can change the order of assembly of the listed features. To move a feature, click on the double arrow at the left of the line. Holding down the mouse button, drag the feature to its new position in the list, and then release the mouse button.
As the line is dragged, a dashed outline follows the cursor, and an arrow at the left of the list shows where the insertion position will be if the mouse is released.
From the mRNA Feature Table drop-down menu, choose which features should be copied into the transcribed sequence: Copy all features, Default, or Do not copy features.
Type a name for the transcribed sequence in the mRNA Sequence Name text box.
Select OK to perform the transcription and display the resulting mRNA sequence.

How do I generate a transcript of a DNA sequence.

Related Topics.