Automated design of primers for Gibson cloning with MacVector.
(View full size on website...)
- Go to FILE | NEW GIBSON/LIGASE INDEPENDENT ASSEMBLY
- Choose the strategy
- Add a vector (from your own digest or the prepared library)
- Drag a feature (e.g. a gene) from your sequence.
- Add a second (or more) fragment.
- Allow MacVector to automatically design primers, or choose your own.
- Check the coding region around junctions to confirm the correct frame is used for protein fusions. You can add spacer residues to adjust the frame.
- Click Assemble to create the final molecule.
Related Topics.
Click Cloning
How do I? - videos
How to digest and ligate fragments into a vector
End modification
Gateway and TOPO cloning
Restriction enzyme
Resetting the origin of a circular sequence.
Automatic Restriction Enzyme Analysis
