How do I map NGS data against a reference genome

Assembler allows you to easily map unpaired or paired sequencing reads against one or more reference sequences:

1. FILE | NEW | ASSEMBLY PROJECT
2. Drag and drop a FASTQ file of sequencing reads into your project.
3. click ADD REF to add your reference sequence.
4. Select both your reference sequence and the sequencing reads file.
5. click ASSEMBLY > Minimap2.
6. Select an appropriate PRESET for your sequencing reads.
7. Click OK.

See the Minimap2 help topic for more details.

Related Topics.

Align to reference.

How do I? - videos

Assemble reads against a reference sequence with Bowtie2.

Assemble reads against a reference sequence with Minimap2.

SNP Report

Editing assemblies

Saving assemblies

Consensus