How do I design primers with tails and mismatches.

MacVector's QuickTest Primer tool gives great flexibility for designing primers with tails or mismatches.

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1. Open a sequence
2. Select 20 bp approximately where your primer should be.
3. ANALYZE | QUICKTEST PRIMER.
4. Slide your primer until the secondary structure is minimal.
5. Type your tail's sequence.
6. Optimize secondary structure again.

See the Primer Design - Overview help topic for more details.

Related Topics.

Primer Design - Overview

How QuickTest Primer scores hairpin loops and primer dimers.

How do I? - videos

Adding primers to the Primer Database

Sequencing primers/probes

Test sequencing primer/probe