How do I create a de novo assembly (SPAdes)

SPAdes (St Petersburg genome assembler) is a de novo assembler intended for sequencing reads from many different sources. It has been designed to handle Illumina and IonTorrent reads. However, it will also do hybrid assemblies with PacBio and Oxford Nanopore and Sanger reads.

1. FILE | NEW | ASSEMBLY PROJECT
2. Drag and drop a FASTQ file of sequencing reads into your project.
3. click SPAdes.

See the SPAdes help topic for more details.

Related Topics.

Assembler

Quick Start

Assembling sequences

Short Read Assembly

Base calling

SPAdes; de novo assembly

Align to reference.

How do I? - videos

Saving assemblies

Consensus