MacVector's QuickTest Primer tool gives great flexibility for designing primers with tails or mismatches.
(View full size on website...)
- Open your template sequence
- Go to ANALYZE | PRIMER DESIGN/TEST(PAIRS).
- For the left primer click USE THIS Primer
- Type or paste in your forward primer
- Repeat for the reverse primer
- Click TEST
See the Primer Design - Overview help topic for more details.
Related Topics.
Primer Design - Overview
How QuickTest Primer scores hairpin loops and primer dimers.
How do I? - videos
Adding primers to the Primer Database
Sequencing primers/probes
Test sequencing primer/probe
