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How do I translate all CDS features in one or more sequences.

There are two new tools that will translate all CDS features in a sequence, or translate every CDS feature in a folder of sequences.

Translate all CDS features..

There is a new Analyze | Translate All CDS Features… menu item that lets you batch translate all CDS features in the active sequence. You might typically use this with an entire annotated bacterial genome, though it works just as well with eukaryotic sequences. There are options to display (and then copy or save) all of the translated proteins in fasta format, or to create a codon usage table (“.bias”) from the results.)

How to translate all CDS features in a sequence.

  1. Open a sequence that has annotated CDS features.
  2. Analyze | Translate All CDS Features…
  3. Toggle Create Codon Usage Table (.bias) on if you also want to generate a custom Codon Usage Table (CUT/.bias) file.
  4. Click OK.

Translate all CDS Features in Folder

There is a new Database | Translate All CDS Features in Folder menu option that is similar to Translate All CDS Features except that it takes a source folder and then loads every sequence file in the folder and translates each CDS feature that it finds, accumulating the results and offering the same result options as Translate All CDS Features. A codon usage viewer window is always created and displayed when you select this option (see below).

How to translate all CDS features in a folder of sequences.

  1. Run Database | Translate All CDS Features in Folder
  2. Choose a folder of sequences you want to translate.
  3. A Results window will appear as well as the Codon Usage Table viewer.
  4. The translated protein sequences are in FASTA format and can be copy and pasted.

Related Topics.

Symbol editor

Editing the general map appearance

How do I? - videos

Generating custom Codon Usage Tables (.bias)