How do I translate a sequence.

Choose Analyze | Translation.
The Translation Analysis dialog box is displayed. If you do not want to translate the entire sequence, select the parts of the sequence to translate, by doing one of the following:
1. type the required ranges in the Segment(s) to translate text box
2. select the required features from the drop-down menu to the right of the box.
3. When you are typing ranges, there must be a slash (/) between the numbers of a range, and a semicolon (;) after each specified range. For segments on the minus (reverse complement) strand, type the base numbers with reference to the plus strand.
4. NOTE: If two or more segments are specified, they must be listed in the order that they are to be translated. If several segments on the minus strand are specified, the first segment to be translated will be the segment with the highest number.
Choose the genetic code to use for the translation from the genetic code drop-down menu.
Choose the reading frame from the phase drop-down menu.
The reading frame is relative to the start of the first segment entered in the Segment(s) to translate box. In almost all cases, the phase should be set to 1.
Choose which strand to translate from the strand drop-down menu. If regions are entered from the features table, the strand is set automatically to the strand corresponding to the last feature that was used.
To create a new protein sequence window containing the protein sequence specified by the translation settings, select the Create new protein check box. Type a name for the protein sequence in the text entry box.The protein sequence will only include the translations of the specified segments.
To generate a table displaying the frequency of each of the 64 codons in the selected sequence, select the Codon Usage Table check box.
Select the Annotated sequence with translation check box to generate an annotated sequence of the DNA and translated protein. This always displays the portion of the nucleic acid sequence specified in the Region to Display text boxes. The displayed sequence can be further controlled as follows:
1. select the as specified in... radio button to see the translation only for those segments specified in the Segment(s) to translate box
2. select the of entire display region radio button to see the translation of the whole display region. With this option, you can choose the number of reading frames translated from the no. of frames drop-down menu.
You can restrict the display to a certain region of the sequence by typing in the base numbers that bracket the region in the Region to Display text boxes, or by selecting a region from the features table drop-down menu at the right of the text boxes. This enables you to display more than just the translated portion of the sequence, for example, if you want to show regulatory regions upstream from the coding region.
Select OK to perform the translation and display the requested results.

How do I translate a sequence.

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