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How do I?

Cloning

How do I check the orientation of a ligated fragment.

How do I subclone digested fragments into a vector

How do I design Gibson Assembly/LIC strategies.

How do I determine RE sites for cloning.

How do I show/hide the RE Picker tool?

Misc.

How do I import Genbank data from a web browser

How to do an online keyword search for sequences.

How do I bring sequences into MacVector.

How do I optimize codon usage.

How do I generate a transcript.

How do I translate a sequence.

Annotation

How do I automatically annotate blank sequences or change the style of a sequence map.

How do I display missing features, predicted ORFs and restriction sites on my sequence.

How do I annotate a gene feature to my sequence.

How do I change the default appearance of features.

How do I import Features from a Genome Browser.

Primer design

How do I add a primer to the Primer Database

How do I design primers with tails and mismatches.

How do I design a pair of primers to amplify a gene?

How do I design a primer to match an existing primer

How do I make a permanent note of where my primer binds on a sequence

How do I test a pair of primers?

Assembly

How do I quickly align sequencing reads against a reference sequence.

How Do I Map NGS data against a reference genome

How do I create a de novo assembly using Velvet

How do I create a de novo assembly using SPAdes

How do I assemble PacBio and NanoPore reads using Flye

How Do I start an Assembly Project

How do I finish a genome assembly

How do I extract specific reads from a FASTQ dataset.

How do I do compare read coverage of different reference assemblies?

Alignments

How Do I identify important differences between two genomes

How Do I visually align a pair of sequences.

How do I do an online BLAST search.

How Do I choose the right alignment tool.