The Primer database allows you to store your own collection of primers. It uses a similar mechanism to subsequence searches and shares a common file format.
Primer Database support mismatches and storing restriction site tails.
The Primer Database tool allows you to save and retrieve primers from subsequence files within Primer3 and Quicktest Primer. You can easily create your own primer database, use existing files or import primers from Excel.
Primers that are saved from within the Design Primers (Primer3) interface or the QuickTest Primers interface will automatically have zero mismatches allowed in the last three bases of the 3' end of the primer.
This tool lets you scan sequences for potential primer binding sites against the Primer Database. Note any stored tails are ignored when searching for binding sites.
How do I add a primer to the Primer Database