You can choose a set of enzymes to use for a site analysis. This may be useful, for example, when you want to restrict your analysis to those enzymes readily available in your laboratory.
1. Open the required enzyme file, and ensure that the file window is active.
A selection of enzyme files is available in the restriction Enzyme folder.
2. If the file is locked, unlock it by toggling the lock button.
3. Scroll through the enzyme list to find the name of each enzyme you want to select.
TIP: You can navigate through the list by typing the first character of the restriction enzyme, or by using the up and down arrows on the keyboard.
4. Click in front of each required name.
A check mark appears, and a line at the bottom of the window indicates how many enzymes have been selected.
5. If you now save the file to disk, using File | Save, your selections will be saved and you will not have to repeat the selection process every time you open this file.
NOTE: When you perform a restriction or proteolytic site search, you may use either all entries in the enzyme file, or only those enzymes selected.
TIP: If you save the changes using File | Save As, you can save different selection subsets.
Searching for restriction sites